This study was carried out to determine the storage, cryotolerance, heat and drying resistance when theL. acidophilus growing at optimal temperature was changed growth temperature and to apply it to probioticsproduction so as to help intensify the viability of lactic acid bacteria in the products. L. acidophilus CT 01was changed temperature at 22℃ and 28℃ after incubated at 37℃ for 18hours. The viable cell numberincreased to 4.9×108 and 5.0×108 cfu/mL, respectively in the changed temperature groups, but decreasedin the control, rapidly down to 7.4×105 cfu/mL in 36 hours. L. acidophilus CT 01 was stored at 4℃ for 12days after changed temperature at 22℃ and 28℃ for 48hours, changed temperature groups had a survivalrate of 112% and 94.43%, higher than that of control (p<0.05). When stored at room temperature, survivalrate in the control was 10.88%, very low, but changed temperature groups had very high survival rate,74.43% and 85.62% (p<0.05). The cryotolerance of L. acidophilus CT 01 appeared higher than changedtemperature groups was observed at 37℃ for 66hours. L. acidophilus CT 01 was heat treated at 60℃ for 15minutes and 30 minutes to examine heat resistance after changed temperature, the heat resistance of L.acidophilus CT 01 appeared higher than control was observed changed temperature groups. L. acidophilus CT01 was inoculated by 30% to the carrier after changed temperature, and dried at 50℃ for 12 hours. Thehighest survival rate of L. acidophilus CT 01 was observed changed temperature groups.