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ISSN : 1225-2964(Print)
ISSN : 2287-3317(Online)
Annals of Animal Resource Sciences Vol.15 No.1 pp.32-36

한우 체외수정란의 수정란이식에 관한 연구

조명현, 박동헌, 장현용, 정희태, 박춘근, 김정익, 양부근*
강원대학교 동물자원과학대학

Studies on the Embryo Transfer of in Vitro Fertilized Embryos in Hanwoo

Boo-Keun Yang*, Myung-Hyun Cho, Dong-Heon Park, Hyun-Young Jang, Hee-Tae Cheong, Choon-Keun Park, Choung-Ik Kim
College of Animal Resource Sciences, Kangwon National University


These studies were carried out to improve the reproductive efficiency through embryos transfer ofHanwoo IVM/IVF embryos. Following routine IVM/IVF procedure, Oocytes and zygotes were cultured for40 to 44 h in CR1aa medium with BSA. Then 2 to 8-cell embryos were removed the cumulus cell and werecultured in CR1aa medium containing 10% fetal bovine serum and 2.5 mM taurine in 5% O2 and 5% CO2at 38.5℃. The fresh and frozen thawed embryos of the morulae and blastocysts cultured for 6 to 9 daysin vitro were transferred into recipients. The pregnancy rates of the blastocyst produced for 6, 7, 8, and9 days in in vitro culture were 41.9, 48.6, 57.9 and 47.4%, respectively. In the developmental stage,pregnancy rates of early blastocysts (41.7%), blastocysts (57.6%) and expanded blastocysts (50.0%) werehigher than that of morulae stage (00.0%). Fresh and frozen embryos on the pregnancy rates were 48.9 and50.0%, respectively. These results indicate that the pregnancy rate after transfer were affected on embryonicstage of in vitro embryos and in vitro culture periods.