ISSN : 1225-2964(Print)
ISSN : 2287-3317(Online)
ISSN : 2287-3317(Online)
한우 체외수정란의 수정란이식에 관한 연구
Studies on the Embryo Transfer of in Vitro Fertilized Embryos in Hanwoo
Abstract
These studies were carried out to improve the reproductive efficiency through embryos transfer ofHanwoo IVM/IVF embryos. Following routine IVM/IVF procedure, Oocytes and zygotes were cultured for40 to 44 h in CR1aa medium with BSA. Then 2 to 8-cell embryos were removed the cumulus cell and werecultured in CR1aa medium containing 10% fetal bovine serum and 2.5 mM taurine in 5% O2 and 5% CO2at 38.5℃. The fresh and frozen thawed embryos of the morulae and blastocysts cultured for 6 to 9 daysin vitro were transferred into recipients. The pregnancy rates of the blastocyst produced for 6, 7, 8, and9 days in in vitro culture were 41.9, 48.6, 57.9 and 47.4%, respectively. In the developmental stage,pregnancy rates of early blastocysts (41.7%), blastocysts (57.6%) and expanded blastocysts (50.0%) werehigher than that of morulae stage (00.0%). Fresh and frozen embryos on the pregnancy rates were 48.9 and50.0%, respectively. These results indicate that the pregnancy rate after transfer were affected on embryonicstage of in vitro embryos and in vitro culture periods.
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