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ISSN : 1225-2964(Print)
ISSN : 2287-3317(Online)
Annals of Animal Resource Sciences Vol.18 No.1 pp.128-132

미니돼지의 체외수정에 영향을 미치는 정자처리의 요인

안신혜, 이유미1), 정희태1), 양부근, 이동석, 박춘근*
강원대학교 동물생명과학대학, 강원대학교 수의학부대학1)

Effect of Sperm Treatment Factors on In Vitro Fertilization of Miniature Pig

Choon-Keun Park*, Shin-Hye Ahn, Yu-Mi Lee1), Hee-Tae Cheong1), Boo-Keun Yang, Dong-Seok Lee
College of Animal life Sciences, Kangwon National University
School of Veterinary Medicine, Kangwon National University1)
(Received: 2007.11.8, Accepted: 2007.12.19)


This study was conducted to investigate the effect of periods of sperm preincubation, concentrations andstorage periods of miniature pig sperm on in vitro penetration of porcine follicular oocytes. High concentration(1×105, 2.5×105, 5×105, 1×106, 5×106 and 1×107) did support sperm penetration than low concentrations (P<0.05).However, polyspermic oocyte rates were increased with high concentrations of sperm. On the other hand, spermpreincubated during 1, 2 or 5h could be penetrated than sperm preincubated during 0, 3 or 4h (P<0.05). Whensperm were storaged with different periods, in vitro pentration rates were significantly higher 0~2 days than3~4 days of sperm storage (p<0.05). These results indicate that sperm treatment factors can effect in vitropenetration in miniature pig.