The aim of this study was to evaluate the effects of orvus es paste(OEP) on the sperm characteristics duringfreezing in boar semen. Semen quality was evaluated the motility, membrane integrity, mitochondria function,acrosome status, viability and abnormality. Boar semen were frozen until 5℃ for 2 hours using cell freezer andmaking the straws, and then freezing by lowing the straws into styrofoam box on the 8cm above the LN2 andplunged into LN₂ for cryopreservation.
In different concentration of OEP (0, 0.25, 0.5 and 1.0%) into cryo-extender, sperm motility, membrane integrity,acrosome status, viability and mitochondria function were significantly higher in 0.5% OEP than those of anyother groups, but sperm abnormality were highest in 1.0% OEP group among all treatment groups (P<0.05). Inthe relationships of the evaluation methods for sperm viability, CBB vs membrane integrity, CBB vs HO/PI andCBB vs mitocondria function were positively correlated (0.67~0.92). Among the evaluation methods, CBB vsmembrane integrity, CBB vs HO/PI and CBB vs mitocondria function were significantly correlated (P<0.001).
These results of this study indicate that supplementation of 0.5% OEP in boar semen cryo-extender can improvethe semen quality.