This study demonstrates that pork cholesterol levels are reduced in finishing swine fed β-cyclodextrin (βCD).The study subjects were 120 swine fed their respective chow diets containing 0, 5, 7, or 10% βCD for 35consecutive days. Plasma total lipids, triglyceride and total cholesterol of the βCD-treated group weresignificantly lower than those of the control group (p<0.05). The levels of plasma lipid were significantlydecreased by 63.22 mg, 73.98 mg, and 82.12 mg in the finishing swine group fed βCD at 5%, 7%, and 10% ,respectively, compared to those in the control group (p<0.05). When 5, 7, and 10% βCD was administered tofinishing swine, the triacylglyceride levels were decreased by 56.24 mg, 55.48 mg, and 60.02 mg, and totalcholesterol concentration was reduced by 25.05 mg, 27.17 mg, and 30.19 mg, respectively, compared to those inthe control group (p<0.05). Excretion of total steroid significantly (p<0.05) increased with increase of βCDsupplementation. The cholesterol levels of swine back fat, belly, loin, and ham were significantly decreased withincrease of βCD supplementation (p<0.05). The pork cholesterol was significantly (p<0.05) reduced by 15.31% inthe βCD-treated group, compared to that of the control group. These results suggest that feeding βCD tofinishing swine may produce novel functional pork with low cholesterol levels.