This study examined the effects of different levels of evening primrose oil(EPO) on the accumulation of γ-fattyacids in broiler meat. Six hundred one-day-old male chicks (Ross strain) from commercial broilers were dividedrandomly into 6 groups × 4 repeat pens. The broilers were fed experimental diets containing 4.0% tallow(control),0.5% EPO, 0.7% mixed oil(EPO 70 : soy bean oil 30), 1.5% EPO, 3.0% EPO or 4.0% EPO for 35 days. There wasa significant difference in body weight gain between the control and treatment groups except for the 0.5% EPOgroup (p<0.05). There was a significant difference in the percentage of thigh and breast weight against the carcassweight between control and treatment groups except for the 0.5% EPO group in the thigh and 0.5% EPO and4.0% EPO groups in the breast weight (p<0.05). The saturated fatty acid levels of the skin and breast muscle lipidof the broilers fed diets containing EPO were significantly lower than that of the control group (p<0.05), whilethe level of unsaturated fatty acid was significantly higher than that of the control group (p<0.05). The γ-fattyacid(GLA, gamma-linolenic acid, 18:3n-6) level was particularly higher in the chicken meat lipids from the broilersfed EPO than in the control group (p< 0.05). This shows that feeding EPO to chicks can produce novel functionalbroiler meat that is enriched in gamma-linolenic acid.