A study was conducted to investigate the efficiency of recipient for Embryo Transfer (ET) in HolsteinHeifers. A total of 193 heifers (Age 14-20 months and body weight, 250-400 Kg) was assigned in 2 groups(natural ovulation cycle group and Hormone-induced ovulation synchronization group) based on thedevelopment of corpus luteum (CL) and uterus where 28.49% (55/193) heifers were in normal estrous. The ETtechnique was applied in both of the groups to transfer the embryo in the recipients and evaluated theirefficiency. In vivo frozen embryos were used for ET at the blastocyst stage. Results showed that according torecipient preparation method the conception rates were 22.72% (10/44) and 40.26% (60/149) in hormone-inducedovulation synchronization group and natural ovulation cycle group, respectively. The pregnancy rate of heiferswas significantly higher (p<0.05) during the first time ET compared to repeat ET; however, recipient showed nosignificant difference in CL development in both side. The conception rate were 31.03% (9/29), 37.75% (57/151)and 15.38% (2/13) at day 6, 7 and 8, respectively after the CL development of the heifers. The conception ratewas significantly higher (p<0.05) in the right side compared to left side of the CL development. In addition,during ET anesthesia group and non anesthesia group conception rate of the recipient were 27.63% (21/76) and41.88% (49/117), respectively.