Selection based on phenotype in the traditional manner does not help in tracking the selected gene.Molecular genetics has revolutionized the old established breeding techniques. A new epoch of molecularmarkers has been acquainted for genetic improvement of livestock. This study is engaged on the neotericmolecular markers used in various fields of livestock. DNA markers are more encouraging in selectinggenomes that have recombination events close to the target gene. Molecular markers rely on DNA assay andare better than the morphological and biochemical markers. In this study DNA-based molecular markersdeveloped during the past decagons for animal genome analysis are reviewed. Mapping of molecularmarkers provide a framework, required for its subsequent use in the selection procedure. Moleculartechniques help in the utilization of genetic variability in breeding population. At the same time livestockgenomes play important role in human genomics and their role for understanding human genomics cannotbe overlooked. Recently, in the epigenetic and transcriptomic studies, RNA sequencing as a part of nextgeneration sequencing has revolutionized the approach and ongoing trends of analysis. Keeping in mind thegoals, these molecular techniques can be implemented successfully by following well defined, crisp andintegrated strategy.