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ISSN : 1225-2964(Print)
ISSN : 2287-3317(Online)
Annals of Animal Resource Sciences Vol.30 No.2 pp.87-96

Detection of Korean Foot-and-Mouth Disease Virus Type A by Immuno-PCR

Kyeongrok Kwak1#,Jeong Keun Lee2#,So-young Choi1,Choon Hong Hwang2,Sung-Jin Lee1*
1College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea
2Dowgene, Seoul 08501, Korea
#These authors contributed equally in this manuscript as first author.
* Corresponding author: Sung-Jin Lee, College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea. Tel: +82-33-250-8636, E-mail:


In the case of foot-and-mouth disease (FMD), there is a great deal of impact on the national economy due to the disposal of diseases, the cost of disease control such as vaccination, reduction of productivity, and restriction of international trade of livestock products. Therefore, appropriate diagnostic methods for sensitive, accurate and rapid identification of virus serotypes are continuously required in terms of early prevention of FMD. This study was conducted to confirm the feasibility of immuno-PCR diagnostic method for the more sensitive detection of Korean FMD virus (FMDV). We synthesized a partial FMD type A viral gene. Protein antigen, monoclonal and polyclonal antibodies of FMDV were cloned, expressed and purified and then magnetic particles were attached to polyclonal antibodies and and oligomers to monoclonal antibodies for the immnuno-PCR. We confirmed the antigen-antibody and oligomer reaction using ELISA, Western blot, and real-time PCR. These results show that Korean FMDV can be detected by using difference of Ct values between positive group and negative group using immuno-PCR.. The results of this study also suggest that this technique will be the basis of the diagnosis method to detect Korean FMDV more sensitively in the future.

Immuno-PCR을 이용한 한국형 A형 구제역 바이러스 진단

강원대학교 동물생명과학대학1, ㈜다우진유전자연구소2