Journal Search Engine
Search Advanced Search Adode Reader(link)
Download PDF Export Citaion korean bibliography PMC previewer
ISSN : 1225-2964(Print)
ISSN : 2287-3317(Online)
Annals of Animal Resource Sciences Vol.31 No.2 pp.52-61

Different Pore Size of Filter Bags Containing Feed Samples Changed Rumen Fermentation Characteristics in an in Vitro System

Joon-Beom Moon1,Han-Been Kim1,Jeong-Soo Lee1,Joong-Kook Park4,Dong-Eun Shin4,Byung-Ki Park4,6,Jae-Yong Song5,Se-Young Lee3,Ja-kyeom Seo2*
1Graduate Students, 2Professor, Department of Animal Science, Pusan National University, Miryang 50463, Korea
3Professor, Department of Animal Science, Yonam College, Cheonan 31005, Korea
4Researcher, Nonghyupfeed Co., Ltd, Seoul 05398, Korea
5Researcher, Institute of Livestock, Nonghyup Co. Ltd., Ansung 17558, Korea
66Professor, Department of Animal Science, Kangwon National University, Chuncheon 24341, Korea
*Corresponding Author: Jakyeom Seo, Life and Industry Convergence Research Institute, Department of Animal Science, Pusan National University, Miryang 50463, Korea. Tel: +82-55-350-5513


In in vitro fermentation studies, feed samples can either be included in the in vitro rumen medium using filter bags or can be directly dispersed. The objective of this study was to investigate the effects of different pore sizes of filter bags on the rumen fermentation characteristics in an in vitro system. Corn, soybean meal, and timothy were ground to pass through a 1.0-mm screen and were formulated in the ratio of 70:7:23 based on DM, respectively. The formulated experimental diet (2g/DM) was put in F57 filter bags and R510 nylon bags (Ankom®) which pore sizes were 25 and 50 μm, respectively. An in vitro study was conducted to determine the rumen fermentation characteristics for 3, 6, 12, 24, and 48 h and rumen microbial community at 48 h of incubation. A significantly higher production of gas was observed in the R510 bags than in F57 at all the incubation times (p<0.01). IVDMD (p<0.01) and IVNDFD (p<0.01) were significantly higher, whereas pH (p<0.01) and NH3-N (p<0.01) were lower when R510 bags were used. In the VFA composition, acetate and butyrate were significantly higher (p<0.01) in R510 bags, and propionate and total VFA concentration did not differ (p=0.55 and 0.25, respectively) between F57 and R510 bags. The log copy numbers of bacteria and protozoa did not differ (p=0.69 and 0.94, respectively) between F57 and R510 bags, whereas those of fungi were significantly higher in R510 than in F57 bags (p<0.01). Therefore, the use of R510 may reflect actual rumen fermentation characteristics more precisely than those of F57 because increased gas production, nutrient digestibility and acetate, butyrate proportion were founded in R510.

사료 주머니의 공극 크기가 in vitro 반추위 내 발효성상에 미치는 영향

부산대학교 생명자원과학대학 동물생명자원과학과 대학원생1, 교수2, 천안연암대학교 축산계열 교수3, 농협사료 연구원4, 농협중앙회 축산연구원5, 강원대학교 동물생명과학대학 교수6