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ISSN : 1225-2964(Print)
ISSN : 2287-3317(Online)
Annals of Animal Resource Sciences Vol.31 No.2 pp.72-81
DOI : https://doi.org/10.12718/AARS.2020.31.2.72

Antioxidant Effects of Fermented Milk Added with Enterococcus faecalis EF-2001 Heat-Killed Probiotics

Hyo-Jin Kang1,Tae-Woon Kim1,Jin-Woo Jhoo2,Gur-Yoo Kim2*
1Graduate Students, 2Professors, Department of Applied Animal Science, College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea
* Corresponding author: Gur-Yoo Kim, Department of Applied Animal Science, College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea. Tel : +82-33-250-8647

Abstract

The creation of probiotic-containing fermented milk products for use by human is an important research topic and has high potential for development. Also, studies have shown that heat-killed probiotics are more stable and easier to use than live probiotics. However, as of this time, research has not been reported in Korea that has evaluated the product or functionality of fermented milk after the addition of heat-killed probiotics. This study was conducted to verify the physiological activity of heat-killed Enterococcus faecalis EF-2001 after its addition to Lactobacillus-fermented milk. Briefly, NFM normal fermented milk (NFM) was used as the control sample, whereas fermented milk with 100 μg/mL EF-2001 (EFM1) and fermented milk with 500 μg/mL EF-2001 (EFM2) were used as the treated samples. Among the samples, EFM2 had the highest acidity of 1.15, but no other factors significantly differed (p<0.05). Furthermore, EFM2 had the highest Lactobacillus count of 9.22 (p<0.05). ABTS, DPPH and FRAP were measured to determine the antioxidant activity of the samples. With respect to those parameters, EFM2 had the highest antioxidant measurements. Therefore, the study confirmed that the addition of E. faecalis EF-2001 to NFM is suitable with the standard and does not affect the quality of characteristics. In conclusion, the treatment sample had higher antioxidant activity than did NFM; this result may be used as a basic for further research and as a guideline for the manufacturing of heat-killed probiotic-containing NFM.

Enterococcus faecalis EF-2001 유산균 사균체 첨가 발효유의 항산화 효과

강효진1,김태운1,주진우2,김거유2*
강원대학교 동물생명과학대학 동물응용과학과 대학원생 1, 교수2

초록

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