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ISSN : 1225-2964(Print)
ISSN : 2287-3317(Online)
Annals of Animal Resource Sciences Vol.33 No.1 pp.150-158
DOI : https://doi.org/10.12718/AARS.2022.33.1.150

Cloning Efficiency of Porcine Somatic Cell Nuclear Transfer Compared with Fetal Fibroblasts and Mesenchymal Stem Cells

Hyo-Jung Kim1, Min Ju Kim1,4, Soo-Hyun Park1, Ye Eun Joo1,4, Chang-Gi Hur3, Bo-Woong Sim4, Myunghoo Kim2, Jakyeom Seo2, Byeong-Woo Kim2, Byung-Wook Cho2, Taek- Soon Shin2 and Seong-Keun Cho2*
1Graduate Students, 2Professor, Department of Animal Science, College of Natural Resources & Life Science, Life and Industry Convergence Research Institute (LICRI), Pusan National University, Miryang 50463, Korea. 3Researcher, CRONEX, Inc.,
Hwaseong 18525, Korea, 4Researcher, Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 28116, Korea

Abstract

Somatic cell nuclear transfer (SCNT) in pigs has been used as a very important tool to produce transgenic for the pharmaceutical protein, xenotransplantation, and disease model and basic research of cloned animals. However, the production efficiency of SCNT embryos is very low in pigs and miniature pigs. The type of donor cell is an important factor influencing the production efficiency of these cloned pigs. Here, we investigated the developmental efficiency of SCNT embryos to blastocysts and full term development using fetal fibroblasts (FF) and mesenchymal stem cells (MSCs) to identify a suitable cell type as donor cell. We isolated each MSCs and FF from the femoral region and fetus. Cultured donor cell was injected into matured embryos for cloning. After that, we transferred cloned embryos into surrogate mothers. In term of in vitro development, the SCNT embryos that used MSCs had significantly higher in cleavage rates than those of FF (81.5% vs. 72%) (p<0.05), but the blastocyst formation rates and apoptotic cell ratio was similar (15.1%, 6.18% vs. 20.8%, 9.32%). After embryo transferred to surrogates, nine and nineteen clone piglets were obtained from the MSCs and FF group, respectively, without significant differences in pregnancy and birth rate (50%, 40% vs. 52.3%, 45.4%) (p>0.05). Moreover, there was no significant difference in the corpus hemorrhagicum numbers of ovary, according to pregnancy, abortion, and delivery of surrogate mothers between MSCs and FF groups. Therefore, the MSCs and FF are useful donor cells for production of clone piglets through SCNT, and can be used as important basic data for improving the efficiency of production of transgenic clone pigs in the future.

태아섬유아세포와 중간엽줄기세포를 이용한 체세포 복제돼지 생산 효율

김효정1 ․ 김민주1,4 ․ 박수현1 ․ 주예은1,4 ․ 허창기3 ․ 심보웅4 ․ 김명후2 ․ 서자겸2 ․ 김병우2 ․ 조병욱2. 신택순2 ․ 조성근2*
부산대학교 생명자원과학대학 동물생명자원과학과 대학원생1, 교수2 (주)크로넥스 연구원3 한국생명공학연구원 연구원4

초록

 

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